Apply Adhesive Plate Sealer
To maximize the use of plates, an adhesive plate-sealing sheet is including in each kit. Using fingers and thumb, adhere firmly to plate. Use a razor to cut sheet and expose wells to be used in assay.
Balance & Spin Vials for PBMC Isolation
Carefully balance the weight of the tubes in centrifuge buckets and spin at 800g for 30 minutes.
Collect Mononuclear Cells
Collect mononuclear cells after density gradient centrifugation.
Create Humidified Chamber
After coating the plate with Capture Solution, incubate plate at 4°C overnight in humidified chamber.
CTL BioSpot Yeast Viability Software Demo
Quick overWatch of CTL BioSpot Yeast Viability Software for counting live and dead cells.
CTL Cell Counting Software Demo
High-throughput image analysis of individual cells using fluorescence detection. Determines viability of mammalian and yeast cells by the individual analysis of live, dead, and apoptotic cells.
CTL TVA™ Software Demo
A demo of the CTL Cell Counting Software in the NK-TVA module.
Diluting Blood Before Isolating PBMC
Diluting the blood before density gradient centrifugation to isolate PBMC for use in an ELISPOT assay.
Ficoll Separation Method #1
Isolation of PBMC by density gradient centrifugation; Ficoll overlay, Method #1.
Ficoll Separation Method #2
Isolation of PBMC by density gradient centrifugation; Ficoll overlay, Method #2
Ficoll Separation Method #3
Isolation of PBMC by density gradient centrifugation, Ficoll underlay, Method #3.
Live/Dead/Apoptotic CellCounting Software
A tool for analyzing the viability and counting live/dead (L/D) and live/dead/apoptotic (L/D/A) peripheral blood mononuclear cells (PBMC) or yeast cells.
NK Target cell Visualization: TVA
The CTL TVA™ is a fast, simple, non-radioactive, direct-imaging-based Natural Killer Assay.
PBMC, Cryopreserved Peripheral Blood Mononuclear Cells
Streamline research with our extensive library of cryopreserved PBMC with >90% post-thaw viability and functionality comparable to fresh. Available either uncharacterized, characterized, HLA-typed, immunotyped, genotyped, and NK reactivity.
Plating Cells & Tapping the Plate
Plate PBMC 100ɥl/well using large orifice tips. After adding cells & antigens, cover plate & gently tap each side.
Prewetting the Plate: ImmunoSpot Assay
Prewetting a 96-well plate for an ImmunoSpot® Enzymatic or FluoroSpot ELISPOT Assay, plus how to fix your plate if it's leaking. Make sure all solutions are freshly-prepared.
Rinse Plate & Remove Underdrain
Stop reaction by rinsing plate under tap water and decanting 3 times;remove protective underdrain and rinse again. Place face down in running hood for 2 hours or on paper towels for 24 hours on bench top.
Store prepared Anti-aggregate Wash Medium in a 37°C CO2 incubator with cap loosened for 20 minutes. This help reset the pH and temperature.
Storing the Cells
After PBMC isolation, cells can be stored for 24 hours in a 37°C incubator with the cap loosened.
Thawing Cryopreserved PBMC
Thawing cryopreserved PBMC and resuspending the cells in CTL Anti-Aggregate™ Wash for use in an ELISPOT assay.
Resuspend PBMC Cell Pellet
Resuspend the mononuclear cell pellet after 2nd spin in centrifuge.
Yeast Viability PPT
PowerPoint presentation about the development and operation of the CTL Yeast Viability Platform.