Our Publications

Home
Resources
Publications

Detection of Antigen-Specific T Cell Lineages and Effector Functions Based on Secretory Signature.

Kirchenbaum GA, Hanson J, Roen DR, Lehmann PV.
J Immunological Sci. 2019, 3, 14-20.

Measuring Th1, Th2, Th17, and Other Effector Lineages - Cytokine Coexpression/Two-color ELISPOT Assays - Cytotoxicity, Granzyme B, and Perforin ELISPOT -

Comprehensive evaluation of the expressed CD8+ T cell epitope space using high-throughput epitope mapping.

Paul V. Lehmann, Maneewan Suwansaard, Ting Zhang, Diana R. Roen, Greg A. Kirchenbaum, Alexey Y.Karulin, Alexander Lehmann, and Pedro A. Reche.
Front. Immunol. 2019, April 26

Determinant Mapping/High-throughput Screening - Working with Peptides and Peptide Pools - Infectious and Other Diseases -

Direct detection of T and B memory lymphocytes reveals HCMV exposure that serum antibodies fail to identify.

Fredrik Terlutter, Tobias M. Nowacki, Richard Caspell, Ruliang Li, Krisztian Horvath, Charlotte von Gall, Stefanie Kurten, Srividya Sundararaman, and Paul V. Lehmann.
Cells. 2018, 19, 7.

Transplantation Immunology - Infectious and Other Diseases -

B cells and B cell blasts withstand cryopreservation while retaining their functionality for producing antibody.

Fecher P., Caspell R., Naeem, V., Karulin A.Y., Kuerten S.and P.V. Lehmann.
Cells. 2018, 7, 50

Fresh versus Cryopreserved PBMC -

High-throughput GLP-capable target cell visualization assay for measuring cell-mediated cytotoxicity.

Welter A., Sundararaman S., Li R., Zhang T., Karulin A.Y., Lehmann A., Naeem V., Roen D.R., Kuerten S, and P. V. Lehmann.
Cells. 2018, 7, 35.

Cytotoxicity, Granzyme B, and Perforin ELISPOT - Tumor Immunology -

Reagent Tracker Dyes permit quality control for verifying plating accuracy in ELISPOT tests.

Lehmann, A., Megyesi Z., Przybyla A., and P.V. Lehmann.
Cells. 2018, 3, 7.

Working with Peptides and Peptide Pools -

A positive control for detection of functional CD4 T cells in PBMC: The CPI pool.

Annemarie Schiller, Ting Zhang, Ruliang Li, Andrea Duechting, Srividya Sundararaman, Anna Przybyla, Stefanie Kuerten and Paul V. Lehmann.
Cells. 2017, 6, 47

Working with Peptides and Peptide Pools -

Delayed activation kinetics of Th2 and Th17 vs. Th1 Cells.

Andrea Duechting, A., Przybyla, A., Kuerten, S. and Lehmann, P.V
Cells. 6, 47

Measuring Th1, Th2, Th17, and Other Effector Lineages -

Multi-color FLUOROSPOT counting using the ImmunoSpot® Fluoro-XTM Suite.

Megyesi, Z., Lehmann, P.V., and Karulin A.Y.
Methods in Molecular Biology, 3rd Edition, (CH 10). 2018, 1808, 115-132.

Detecting all Immunoglobulin Classes and Subclasses in Multiplex 7 Color ImmunoSpot® Assay.

Caspell, R., and Paul V. Lehmann.
Methods in Molecular Biology, 3rd Edition, (CH. 8). 2018, 1808, 85-94.

Multiplex ImmunoSpot® Assays for the Study of Functional B Cell Subpopulations.

Roen, D.R., Hanson J, and Lehmann, P.V.
Methods in Molecular Biology, 3rd Edition, (CH.7). 2018, 1808, 73-84

Four color ELISPOT assay for the identification of T cell subpopulations.

Hanson, J. and Lehmann, P.V.
Methods in Molecular Biology,3rd Edition,(CH 5.) 2018, 1808, 51-62

Measuring Th1, Th2, Th17, and Other Effector Lineages -

Multiplexing T- and B-cell FLUOROSPOT assays: Experimental validation of the multi-color ImmunoSpot® Software based on center of mass distance algorithm.

Alexey Y. Karulin*, Zoltán Megyesi, Richard Caspell, Jodi Hanson, and Paul V. Lehmann.
Methods in Molecular Biology, 3rd Edition (CH 9). 2018, 1808, 95-114.

Cognate Cytokine Production by T Cells versus Bystander Cytokine Production -

Secondhand smoke impairs immune responses to respiratory infections.

Tariq A. Bhat, Kalathil Suresh, Paul N. Bogner, Paul V Lehmann, Thomas H. Thatcher, Richard P. Phipps, Patricia J. Sime and Yasmin Thanavala.
J. Immunology. 2018, 15, 2927-2940.

Tumor Immunology -

The enteric nervous system is a potenital autoimmune target in multiple sclerosis.

Marie Wunsch et al, Paul V. Lehmann and Stefanie Kuerten.
Acta Neuropathologica. 2017, 134, 281-295.

Autoimmunity

The correlation between the virus- and brain antigen-specific B cell response in the blood of patients with multiple sclerosis.

Wunsch M, Hohmann C, Milles B, Rostermund C, Lehmann PV, Schroeter M, Bayas A, Ulzheimer J, Mäurer M, Ergün S, and Kuerten S.
Viruses. 2016, 8, 105.

AutoimmunityAutoimmunity

How frequently are predicted peptides actually recognized by CD8 cells?

Ioana Moldovan, Oleg Targoni, Wenji Zhang, and Paul V Lehmann.
Cancer Immunology, Immunotherapy. 2016, 65, 847-855.

Single-cell Resolution and Frequency - Cognate Cytokine Production by T Cells versus Bystander Cytokine Production - Determinant Mapping/High-throughput Screening - Working with Peptides and Peptide Pools - Infectious and Other Diseases -

ELISPOT assays in 384-well format: Up to 30 data points with one million cells.

Hanson J,Sundararaman S, Caspell R, Karacsony E, Karulin AY, and Lehmann PV.
Cells. 2015, 4, 71-83.

Single-cell Resolution and Frequency -

ELISPOTs produced by CD8 and CD4 cells follow Log Normal size distribution permitting objective counting.

Karulin AY, Karacsony K, Zhang W, Targoni OS, Moldovan I, Dittrich M, Sundararaman S, and Lehmann PV.
Cells. 2015, 4, 56-70.

Spot Size Distribution -

Characterization of the HCMV-specific CD4 T cell responses that are associated with protective immunity.

Wunsch M, Zhang W, Hanson J, Caspell R, Karulin AY, Recks MS, Kuerten S, Sundararaman S, and Lehmann PV.
Cells. 2015, 7, 4414-4437.

Single-cell Resolution and Frequency - Measuring Th1, Th2, Th17, and Other Effector Lineages - Cognate Cytokine Production by T Cells versus Bystander Cytokine Production - Cytokine Coexpression/Two-color ELISPOT Assays - Spot Size Distribution - Infectious and Other Diseases -

Normal distribution of CD8+ T cell-derived ELISPOT counts within replicates justifies the reliance on parametric statistics for identifying positive responses.

Karulin AY, Caspell R, Dittrich M, and Lehmann PV.
Cells. 2015, 4, 96-111.

Single-cell Resolution and Frequency -

Serial measurements of apoptotic cell numbers provide better acceptance criterion for PBMC quality than a single measurement prior to the T cell assay.

Wunsch M, Caspell R, Kuerten S, Lehmann PV, and Sundararaman S.
Cells. 2015, 4, 40-55.

Fresh versus Cryopreserved PBMC -

High reproducibility of ELISPOT counts from nine different laboratories.

Sundararaman S, Karulin AY, Ansari T, BenHamouda N, Gottwein J, Laxmanan S, Levine SM, Loffredo JT, McArdle S, Neudoerfl C, Roen D, Silina K, Welch M, and Lehmann PV.
Cells. 2015, 4, 21-39.

Spot Size Distribution - Assay to Assay Reproducibility of Ex Vivo Measurements on PBMC -

Identification of a B cell-dependent subpopulation of multiple sclerosis by measurements of brain-reactive B cells in the blood.

Kuerten S, Pommerschein G, Barth SK, Hohmann C, Miles B, Sammer FW, Duffy CE, Wunsch M, Rovituso DM, Schroeter M, Addicks K, Kaiser CC, Lehmann PV.
Clinical Immunology. 2014, 152, 20-24.

Autoimmunity

Longitudinal T cell-derived IFN-γ/IL-17 balances do not correlate with the disease course in two mouse models of experimental autoimmune encephalomyelitis.

Kuerten S, Wunsch M, Lehmann PV.
Journal of Immunological Methods. 2013, 398-399, 68-75.

Cytokine Coexpression/Two-color ELISPOT Assays - Autoimmunity

An enhanced ELISPOT assay for sensitive detection of antigen-specific T cell responses to Borrelia burgdorferi.

Chenggang Jin, Roen Diana R, Lehmann PV, Kellermann Gottfried H.
Cells. 2013, 2, 607-620.

Infectious and Other Diseases -

Chapter: When results of T cell immune monitoring match or do not match clinical outcomes of tumor vaccine trials — What more could and should we measure?

Lehmann PV, Sundararaman S.
The Tumor Immunoenvironment. (CH-32). 2013, 725-740.

Measuring Th1, Th2, Th17, and Other Effector Lineages - Tumor Immunology -

How much of Virus-Specific CD8 T Cell Reactivity is Detected with a Peptide Pool when Compared to Individual Peptides?

Zhang W, Moldovan I, Targoni OS, Subbramanian RA, Lehmann PV.
Viruses. 2012, 4, 2636-2649.

Single-cell Resolution and Frequency - Measurements of Functional T Cell Avidity - Working with Peptides and Peptide Pools - Infectious and Other Diseases -

How ELISPOT morphology reflects on the productivity and kinetics of cells’ secretory activity.

Karulin AY, Lehmann PV.
Methods and Protocols, Handbook of ELISPOT. 2011, 792, 125-143.

Resting of Cryopreserved PBMC Does Not in General Benefit the Performance of Antigen-specific T Cell ELISPOT Assays.

Kuerten S, Batoulis H, Recks MS, Karacsony E, Zhang W, Subbramanian R, Lehmann, PV.
Cells. 2012, 1, 313-324.

Single-cell Resolution and Frequency - Assay to Assay Reproducibility of Ex Vivo Measurements on PBMC - Fresh versus Cryopreserved PBMC -

Optimal thawing of cryopreserved peripheral mononuclear cells for use in high-throughput human immune monitoring studies.

Ramachandran H, Laux J, Moldovan I, Caspell R, Lehmann PV, Subbramanian R.
Cells. 2012, 1, 313-324.

Fresh versus Cryopreserved PBMC -

Chapter: Statistical analysis of ELISPOT assays.

Dittrich M, Lehmann PV.
Methods and Protocols, Handbook of ELISPOT. 2011, 792, 173-183.

Chapter: Unique strengths of ELISPOT for T cell diagnostics.

Lehmann PV, Zhang W.
Methods and Protocols, Handbook of ELISPOT. 2011. 792, 3-23.

T and B Cell Immunity is not Essential for the Induction of Proliferative Vitreoretinopathy.

Zhang W, Tan J, Liu Y, Li W, Gao Q, Lehmann PV.
Eye. 2012, 26, 872-881.

Autoimmunity

The Magnitude of the Antigen-specific T Cell Response is Separated from the Severity of Spinal Cord Histopathology in Remitting-relapsing Experimental Autoimmune Encephalomyelitis.

Batoulis H, Uhl M, Addicks K, Lehmann PV, Kuerten S.
Glia. 2012, 60, 794-805.

Autoimmunity

Neuroantigen-specific CD4 Cells Expressing IFN-γ, IL-2, and IL-3 in a Mutually Exclusive Manner Prevail in EAE, Representing Independent Th1 Sublineages.

Karulin AY, Quast S, Hesse M, Lehmann PV.
Cells. 2012, 1, 576-596.

Measuring Th1, Th2, Th17, and Other Effector Lineages - Cytokine Coexpression/Two-color ELISPOT Assays - Autoimmunity

Cytomegalovirus (CMV)-specific Perforin and Granzyme B ELISPOT Assays Detect Reactivation of CMV Infection in Inflammatory Bowel Disease.

Nowacki T, Bettenworth D, Ross M, Heidemann J, Lehmann PV, Lügering A.
Cells. 2012, 1, 35-50.

Cytotoxicity, Granzyme B, and Perforin ELISPOT - Infectious and Other Diseases -

Dissecting the T Cell Response: Proliferation Assays vs. Cytokine Signatures by ELISPOT.

Anthony DD, Milkovich KA, Zhang W, Rodriguez B, Yonkers NL, Heeger PS, Tary-Lehmann M, Lehmann PV.
Cells. 2012, 1, 127-140.

Measuring Th1, Th2, Th17, and Other Effector Lineages - Cognate Cytokine Production by T Cells versus Bystander Cytokine Production -

Studies on the CNS histopathology of EAE and its correlation with clinical and immunological parameters.

Kuerten S, Addicks K, Lehmann PV.
Cells. 2012, 1, 576-596.

Autoimmunity

Chapter: Objective, user-independent ELISPOT data analysis based on scientifically-validated principles.

Zhang W, Lehmann PV.
Methods and Protocols, Handbook of ELISPOT. 2011, 792, 155-171.

The Immune Pathogenesis of Experimental Autoimmune Encephalomyelitis – Lessons Learned for Multiple Sclerosis?

Kuerten S and Lehmann PV.
Journal of Interferon & Cytokine Research. 2011. 31:907-16.

Autoimmunity

Myelin-reactive antibodies mediate the pathology of MBP-PLP fusion protein MP4-induced EAE.

Kuerten S, Pauly R, Rottlaender A, Rodi M, Gruppe TL, Addicks K, Tary-Lehmann M, Lehmann PV.
Clinical Immunology. 2011, 140, 54-62.

Working with Peptides and Peptide Pools - Autoimmunity

Differential patterns of spinal cord pathology induced by MP4, MOG peptide 35-55, and PLP peptide 178-191 in C57BL/6 mice.

Kuerten S, Gruppe TL, Laurentius LM, Kirch C, Tary-Lehmann M, Lehmann PV, Addicks K.
APMIS. 2011, 119, 336-346.

Autoimmunity

Efficacy of a Tetravalent Chimeric Dengue Vaccine (DENVax) in Cynomolgus Macaques.

Osorio JE, Brewoo JN, Silengo SJ, Arguello J, Moldovan IR, Tary-Lehmann M, Powell TD, Livengood JA, Kinney RM, Huang CYH and Stinchcomb DT.
American Society of Tropical Medicine and Hygiene. 84(6), 2011. 978–987.

Infectious and Other Diseases -

The Polyomavirus BK large T-antigen-derived peptide elicits an HLA-DR promiscuous and polyfunctional CD4+ T cell response.

Ramaswami B, Popescu I, Macedo C, Luo C, Shapiro R, Metes D, Chalasani G, and Randhawa PS.
Clinical Vaccine Immunology. 2011, 18, 815-824.

Infectious and Other Diseases -

Recommendations from the iSBTc- SITC/FDA/NCI Workshop on Immunotherapy BioMarkers.

Butterfield LH, Palucka AK, Britten CM, Dhodapkar MV, Hakansson L, Janetzki S, Kawakami Y, Kleen TO, Lee PP, Maccalli C, Maecker HT, Maino VC, Maio M, Malyguine A, Masucci G, Pawelec G, Potter DM, Rivoltini L, Salazar LG, Schenedel DJ, Slingluff CL, Song W, Stroncek DF, Tahara H, Thurin M, Trinchieri G, van Der Burg SH, Whiteside TL, Wigginton JM, Marincola F, Khleif S, Fox BA, Disis ML.
Clinical Cancer Research. 2011, 17, 3064-3076.

Tumor Immunology -

The clinical course of EAE is reflected by the dynamics of the neuroantigen-specific T cell compartment in the blood.

Kuerten S, Rottlaender A, Rodi M, Velasco VB Jr, Schroeter M, Kaiser C, Addicks K, Tary-Lehmann M, Lehmann PV.
Clinical Immunology. 2010, 137, 422-432.

ELISPOT for Cells Other Than Human PBMC or Murine Lymph Node/Spleen Cells - Autoimmunity

Involvement of brain-derived neurotrophic factor (BDNF) in MP4-induced autoimmune encephalomyelitis.

Javeri S, Rodi M, Tary-Lehmann M, Lehmann PV, Addicks K, Kuerten S.
Clinical Immunology. 2010, 137, 181-189.

Autoimmunity

Th1 responses to beta-amyloid in young humans convert to regulatory IL-10 responses in Down syndrome and Alzheimer’s disease.

Loewenbrueck KF, Tigno-Aranjuez JT, Boehm BO, Lehmann PV, Tary-Lehmann M.
Neurobiology of Aging. 2010, 31, 1732-42.

Infectious and Other Diseases -

Delineating the impact of neuroantigen vs genetic diversity on MP4-induced EAE of C57BL/6 and B6.129 mice.

Kuerten S, Rodi M, Javeri S, Gruppe TL, Tary-Lehmann M, Lehmann PV, Addicks K.
APMIS. 2009, 117, 923-35.

Autoimmunity

ELISPOT assays provide reproducible results among different laboratories for T-cell immune monitoring – even in hands of ELISPOT-inexperienced investigators.

Zhang W, Caspell R, Karulin AY, Ahmad M, Haicheur N, Abdelsalam A, Johannesen K, Vignard V, Dudzik P, Georgakopoulou K, Mihaylova A, Silina K, Aptsiauri N, Adams V, Lehmann PV, McArdle S.
Journal of Immunotoxicology. 2009, 6, 227-34.

Single-cell Resolution and Frequency - Measurements of Functional T Cell Avidity - Assay to Assay Reproducibility of Ex Vivo Measurements on PBMC -

Impaired plasmacytoid dendritic cell (PDC)-NK cell activity in viremic human immunodeficiency virus infection attributable to impairments in both PDC and NK cell function.

Conry SJ, Milkovich KA, Yonkers NL, Rodriguez B, Bernstein HB, Asaad R, Heinzel FP, Tary-Lehmann M, Lederman MM, Anthony DD.
Journal of Virology. 2009, 83, 11175-11187.

ELISPOT for Cells Other Than Human PBMC or Murine Lymph Node/Spleen Cells - Infectious and Other Diseases -

Encephalitogenicity of complete Freund’s adjuvant relative to CpG is linked to induction of Th17 cells.

Tigno-Aranjuez JT, Jaini R, Tuohy VK, Lehmann PV, Tary-Lehmann M.
Journal of Immunology. 2009, 183, 5654-5661.

Measuring Th1, Th2, Th17, and Other Effector Lineages - Vaccine and Adjuvants - Autoimmunity

Dissociated induction of cytotoxicity and DTH by CFA and CpG (41.8)

Tigno JT, Lehmann PV, Tary-Lehmann M.
Journal of Immunotherapy. 2009, 32, 389.

Measuring Th1, Th2, Th17, and Other Effector Lineages - Cytotoxicity, Granzyme B, and Perforin ELISPOT - Vaccine and Adjuvants -

Accessory cell dependent NK cell mediated PBMC IFN-gamma production is defective in HIV infection.

Yonkers NL, Milkovich KA, Rodriguez B, Post AB, Asaad R, Heinzel FP, Valdez H, Tary-Lehmann M, Anthony DD.
Clinical Immunology. 2009, 131, 288-297.

ELISPOT for Cells Other Than Human PBMC or Murine Lymph Node/Spleen Cells - Infectious and Other Diseases -

Increased per cell IFN-gamma productivity indicates recent in vivo activation of T cells.

Schlingmann TR, Shive CL, Targoni OS, Tary-Lehmann M, Lehmann PV.
Cellular Immunology. 2009, 258, 131-137.

Spot Size Distribution -

Perspective: research highlights at the Case Western Reserve University Center for AIDS Research. Learning from the past and looking toward the future.

Le Grice SF, Whalen CC, Hom DL, Lehmann PV, Walker CJ.
AIDS Research and Human Retroviruses. 1996, 12, 1659.

Infectious and Other Diseases -

Fundamental differences in the dynamics of CNS lesion development and composition in MP4- and MOG peptide 35-55-induced experimental autoimmune encephalomyelitis.

Kuerten S, Javeri S, Tary-Lehmann M, Lehmann PV, and Angelov DN.
Clinical Immunology. 2008, 129, 256-267.

Autoimmunity

The TRAIL of helpless CD8+ T cells in HIV infection.

Kuerten S, Asaad RJ, Schoenberger SP, Lehmann PV, Tary-Lehmann M.
AIDS Research and Human Retroviruses. 2008, 24, 1-9.

Measuring Th1, Th2, Th17, and Other Effector Lineages - Infectious and Other Diseases -

Antigen structure influences helper T-cell epitope dominance in the human immune re- sponse to HIV envelope glycoprotein gp120.

Mirano-Bascos D, Tary-Lehmann M and Landry SJ.
European Journal of Immunology. 2008, 38, 1231-1237.

Infectious and Other Diseases -

Lack of Disease Specificity Limits the Usefulness of in vitro Costimulation in HIV- and HCV-Infected Patients.

Kuerten S, Schlingmann T, Rajasalu T, Angelov DN, Lehmann PV, Tary-Lehmann M.
Clinical and Developmental Immunology. 2008, 38, 1231-1237.

Infectious and Other Diseases -

Chapter: Validating reference samples for comparison in a regulated ELISPOT assay.

Tary-Lehmann M, Hamm CD, Lehmann PV.
Validation of Cell-based Assays in the GLP Setting. (CH 9) 2008.

Making the jump to high throughput microbial detection

Hattier T and Lehmann PV.
Medlab. 2008, 3, 30-32.

Autoimmunity

Allogeneic transplantation of multiple umbilical cord blood units in adults: role of pretransplant-mixed lymphocyte reaction to predict host-vs-graft rejection.

Fanning LR, Hegerfeldt Y, Tary-Lehmann M, Lesniewski M, Maciejewski J, Weitzel RP, Kozik M, Finney M, Lazarus HM, Paul P, Ratajczak MZ, Meyerson HJ, Laughlin MJ.
Leukemia. 2008, 22, 1786-90.

Transplantation Immunology -

Immunogenicity of unprocessed and photooxidized bovine and human osteochondral grafts in collagen-sensitive mice BMC.

Kawalec-Carroll JS, Hetherington VJ, Dockery DS, Shive C, Targoni OS, Lehmann PV, Nadler D, Prins D.
Musculoskeletal Disorders. 2006. 7:32.

Transplantation Immunology -

The secretory IFN-γ response of single CD4 memory cells after activation on different antigen presenting cell types.

Ott PA, Tary-Lehmann M, and Lehmann PV.
Clinical Immunology. 2007, 124, 267-276.

Single-cell Resolution and Frequency -

MP4- and MOG:35-55-induced EAE in C57BL/6 mice differentially targets brain, spinal cord and cerebellum.

Kuerten S, Kostova-Bales DA, Frenzel LP, Tigno JT, Tary-Lehmann M, Doychin N. Angelov, Lehmann PV.
Journal of Neuroimmunology. 2007, 189, 31-40.

Autoimmunity

Kinetics and Organ Distribution of IL-17-Producing CD4 Cells in Proteolipid Protein 139-151 Peptide-Induced Experimental Autoimmune Encephalomyelitis of SJL Mice.

Hofstetter HH, Toyka KV, Tary-Lehmann M, Lehmann PV.
Journal of Immunology. 2007, 178, 1372-1378.

Measuring Th1, Th2, Th17, and Other Effector Lineages - Autoimmunity

Neonatal induction of myelin-specific Th1/Th17 immunity does not result in experimental autoimmune encephalomyelitis and can protect against the disease in adulthood.

Hofstetter HH, Kovalovski A, Shive C, Lehmann PV, Forsthuber TG.
Journal of Neuroimmunology. 2007, 187, 20-30.

Measuring Th1, Th2, Th17, and Other Effector Lineages - Autoimmunity

Granzyme-B production, but not cytokine signatures distinguish recently activated CD8 memory cells from resting memory cells.

Nowacki TM, Kuerten S, Zhang W, Boehm BO, Lehmann PV, Tary-Lehmann M.
Cell Immunology. 2007, 247, 36-48.

Cytotoxicity, Granzyme B, and Perforin ELISPOT -

Dissociation of EAE protective effect and allergic side reactions in tolerization with neuroantigen.

Lichtenegger FS, Kuerten S, Faas S, Boehm BO, Tary-Lehmann M, Lehmann PV.
Journal of Immunology. 2006, 178, 4749-4756.

Measuring Th1, Th2, Th17, and Other Effector Lineages - Autoimmunity

Dissociated production of perforin, granzyme B and IFN-γ by HIV-specific CD8+ cells in HIV infection.

Kuerten S, Kleen T, Assad RJ, Lehmann PV, Tary-Lehmann M.
AIDS Research and Human Retroviruses. 2007, 24, 62-71.

Cytotoxicity, Granzyme B, and Perforin ELISPOT - Infectious and Other Diseases -

HIV Coinfection Impairs CD28-Mediated Costimulation of Hepatitis C Virus-Specific CD8 Cells.

Yonkers NL, Rodriguez B, Post AB, Asaad R, Jones L, Lederman MM, Lehmann PV, Anthony DD.
Journal of Infectious Diseases. 2006, 194, 391-400.

Infectious and Other Diseases -

MBP-PLP fusion protein-induced EAE in C57BL/6 mice.

Kuerten S, Lichtenegger FS, Faas S, Angelov DN, Tary-Lehmann M, Lehmann PV.
Journal of Neuroimmunology. 2006, 177, 99-111.

Measuring Th1, Th2, Th17, and Other Effector Lineages - Autoimmunity

Immunologic testing of xeno-derived osteochondral grafts using peripheral blood mononuclear cells from healthy human donors.

Kawalec-Carroll JS, Hetherington VJ, Dockery DS, Shive C, Targoni OS, Lehmann PV, Nadler D, Prins D.
BMC Musculoskeletal Disorders. 2005, 36.

Transplantation Immunology -

The cytokine signature of MOG-specific CD4 cells in the EAE of C57BL/6 mice.

Hofstetter HH, Karulin AY, Forsthuber TG, Ott PA, Tary-Lehmann M, Lehmann PV.
Journal of Neuroimmunology. 2005, 170, 105-114.

Single-cell Resolution and Frequency - Cognate Cytokine Production by T Cells versus Bystander Cytokine Production - Autoimmunity

Differential effect of interleukin-2 treatment on primary and secondary immunizations in HIV infected individuals.

Kuekrek H, Schlingmann T, Valdez H, Boehm BO, Pollard RB, Mitsuyasu R, Goebel FD, Lederman MM, Lehmann PV, Tary-Lehmann M.
AIDS. 2005, 19, 1967-1974.

Vaccine and Adjuvants - Infectious and Other Diseases -

Recipient-specific tolerance after HLA-mismatched umbilical cord blood stem cell transplantation.

Kleen TO, Kadereit S, Fanning LR, Jaroscak J, Fu P, Meyerson HJ, Kulchycki L, Slivka LF, Kozik M, Tary-Lehmann M, Laughlin MJ.
Transplantation. 2005, 80, 1316-1322.

Measuring Th1, Th2, Th17, and Other Effector Lineages - Transplantation Immunology -

IL-2 absorption affects IFN- gamma and IL-5, but not IL-4 producing memory T cells in double color cytokine ELISPOT assays.

Quast S, Zhang W, Shive C, Kovalovski D, Ott PA, Herzog BA, Boehm BO, Tary-Lehmann M, Karulin AY, Lehmann PV.
Cell Immunology. 2005, 237, 28-36.

Cytokine Coexpression/Two-color ELISPOT Assays -

CD4+CD25+ regulatory T cells control the progression from periinsulitis to destructive insulitis in murine autoimmune diabetes.

Ott PA, Anderson MR, Tary-Lehmann M, Lehmann PV.
Cell Immunology. 2005, 235, 1-11.

Autoimmunity

Phenotypic and functional analysis of EBV-specific memory CD8 cells in SLE.

Berner BR, Tary-Lehmann M, Yonkers NL, Askari AD, Lehmann PV, Anthony DD.
Cell Immunology. 2005, 235, 29-38.

Infectious and Other Diseases -

Does the frequency and avidity spectrum of the neuroantigen-specific T cells in the blood mirror the autoimmune process in the central nervous system of mice undergoing experimental allergic encephalomyelitis?

Hofstetter HH, Targoni OS, Karulin AY, Forsthuber TG, Tary-Lehmann M, Lehmann PV.
Journal of Immunology. 2005, 174, 4598-4605.

Single-cell Resolution and Frequency - Measurements of Functional T Cell Avidity - Autoimmunity

Islet-cell antigen-reactive T cells show different expansion rates and Th1/Th2 differentiation in type 1 diabetic patients and healthy controls.

Ott PA, Herzog BA, Quast S, Hofstetter HH, Boehm BO, Tary-Lehmann M, Durinovic-Bello I, Berner BR, Lehmann PV.
Clinical Immunology. 2005, 115, 102-114.

Measuring Th1, Th2, Th17, and Other Effector Lineages - Cytokine Coexpression/Two-color ELISPOT Assays - Working with Peptides and Peptide Pools - Autoimmunity

Chapter: Image analysis and data management of ELISPOT assay results.

Lehmann PV.
Methods in Molecular Biology. 2005, 302, 117-32.

Spot Size Distribution -

Results of an ELISPOT Proficiency Panel Conducted in 11 Laboratories Participating in International Human Immunodeficiency Virus Type 1 Vaccine trials.

Cox JH, Ferrari G, Kalams SA, Lopaczynski W, Oden N, D’Souza MP and the ELISPOT Collaborative Study Group.
AIDS Research & Human Retroviruses. 2005, 21, 68-81.

Assay to Assay Reproducibility of Ex Vivo Measurements on PBMC - Infectious and Other Diseases -

Increased in vivo frequency of IA-2 peptide-reactive IFNgamma+/IL-4- T cells in type 1 diabetic subjects.

Herzog BA, Ott PA, Dittrich MT, Quast S, Karulin AY, Kalbacher H, Karges W, Tary-Lehmann M, Lehmann PV, Boehm BO, Durinovic-Bello U.
Journal of Autoimmunity. 2004, 23, 45-54.

Working with Peptides and Peptide Pools - Autoimmunity

T cell immunity induced by live, necrotic, and apoptotic tumor cells.

Bartholomae WC., Rininsland FH, Eisenberg JC, Boehm BO, Lehmann PV, Tary-Lehmann M.
Journal of Immunology. 2004, 173, 1012-1022.

Tumor Immunology -

The third signal in T cell-mediated autoimmune disease?

Darabi K, Karulin AY, Boehm BO, Hofstetter HH, Fabry Z, LaManna JC, Chavez JC, Tary-Lehmann M, Lehmann PV.
Journal of Immunology. 2004, 173, 92-99.

Autoimmunity

Selective impairments in dendritic cell-associated function distinguish hepatitis C virus and HIV infection.

Anthony DD, Yonkers NL, Post AB, Asaad R, Heinzel FP, Lederman MM, Lehmann PV, Valdez H.
Journal of Immunology. 2004, 172, 4907-4916.

Infectious and Other Diseases -

Tc1 effector diversity shows dissociated expression of granzyme B and interferon- gamma in HIV infection.

Kleen TO, Asaad R, Landry, Boehm BO, Tary-Lehmann M.
AIDS. 2004, 18, 383-392.

Working with Peptides and Peptide Pools - Cytotoxicity, Granzyme B, and Perforin ELISPOT - Infectious and Other Diseases -

T cells recognize multiple GAD65 and proinsulin epitopes in human type 1 diabetes, suggesting determinant spreading.

Ott PA, Dittrich MT, Herzog BA, Guerkov R, Gottlieb PA, Putnam AL, Durinovic-Bello I, Boehm BO, Tary-Lehmann M, Lehmann PV.
J Clin Immunol. 2004, 24, 327-339.

Working with Peptides and Peptide Pools - Autoimmunity

CD28 costimulation enhances the sensitivity of the ELISPOT assay for detection of antigen-specific memory effector CD4 and CD8 cell populations in human diseases.

Ott PA, Berner BR, Herzog BA, Guerkov R, Yonkers NL, Durinovic-Bello I, Tary-Lehmann M, Lehmann PV, and Anthony DD.
Journal of Immunological Methods. 2004, 285, 223-235.

Working with Peptides and Peptide Pools - Vaccine and Adjuvants -

Deficient IFN-gamma expression in umbilical cord blood (UCB) T cells can be rescued by IFN-gamma-mediated increase in NFATc2 expression.

Kadereit S, Junge GR, Kleen T, Kozik MM, Kaminski BA, um-Woods K, Fu P, Tary-Lehmann M, Laughlin MJ.
Journal of Clinical Immunology. 2003, 23, 485-497.

Transplantation Immunology -

Intratumor CpG-oligodeoxynucleotide injection induces protective antitumor T cell immunity.

Lonsdorf AS, Kuekrek H, Stern BV, Boehm BO, Lehmann PV, Tary-Lehmann M.
Journal of Immunology. 2003, 171, 3941-3946.

Vaccine and Adjuvants - Tumor Immunology -

Protective efficacy of anti-Helicobacter pylori immunity following systemic immunization of neonatal mice.

Eisenberg JC, Czinn SJ, Garhart CA, Redline RW, Bartholomae WC, Gottwein JM, Nedrud JG, Emancipator SE, Boehm BO, Lehmann PV, Blanchard TG.
Infection and Immununity. 2003, 71, 1820-1827

Measuring Th1, Th2, Th17, and Other Effector Lineages - Vaccine and Adjuvants - Infectious and Other Diseases -

Detection of low-frequency antigen-specific IL-10-producing CD4(+) T cells via ELISPOT in PBMC: cognate vs. nonspecific production of the cytokine.

Guerkov RE, Targoni OS, Kreher CR, Boehm BO, Herrera MT, Tary-Lehmann M, Lehmann PV, Schwander SK.
Journal of Immunological Methods. 2003, 279, 111-121.

Cognate Cytokine Production by T Cells versus Bystander Cytokine Production - Measuring Th1, Th2, Th17, and Other Effector Lineages - Infectious and Other Diseases - Spot Size Distribution -

Enzyme linked immunosorbent spot (ELISPOT) assay for interferon-gamma independently predicts renal function in kidney transplant recipients.

Hricik DE, Rodriguez V, Riley J, Bryan K, Tary-Lehmann M, Greenspan N, Dejelo C, Schulak JA, Heeger PS.
American Journal of Transplantation. 2003, 3, 878-884.

Transplantation Immunology -

T-cell epitope mapping using the ELISPOT approach.

Anthony DD, Lehmann PV.
Methods. 2003, 29, 260-269.

Determinant Mapping/High-throughput Screening - Working with Peptides and Peptide Pools - Assay to Assay Reproducibility of Ex Vivo Measurements on PBMC - Infectious and Other Diseases -

CD4+ and CD8+ cells in cryopreserved human PBMC maintain full functionality in cytokine ELISPOT assays.

Kreher CR, Dittrich MT, Guerkov R, Boehm BO, Tary-Lehmann M.
Journal of Immunological Methods. 2003, 278, 79-93.

Working with Peptides and Peptide Pools - Assay to Assay Reproducibility of Ex Vivo Measurements on PBMC - Fresh versus Cryopreserved PBMC -

On the same cell type GPI- anchored normal cellular prion and DAF protein exhibit different biological properties.

Li R, Liu T, Yoshihiro F, Tary-Lehmann M, Obrenovich M, Kuekrek H, Kang SC, Pan T, Wong BS, Medof ME, Sy MS.
Biochemical and Biophysical Research Communications. 2003, 303, 446-451

Infectious and Other Diseases -

Autoreactive T cells promote post-traumatic healing in the central nervous system.

Hofstetter HH, Sewell DL, Liu F, Sandor M, Forsthuber T, Lehmann PV, Fabry Z.
Journal of Neuroimmunology. 2003, 134, 25-34.

Autoimmunity

Evolution of the enzyme-linked immunosorbent spot assay for post-transplant alloreactivity as a potentially useful immune monitoring tool.

Gebauer BS, Hricik DE, Atallah A, Bryan K, Riley J, Tary-Lehmann M, Greenspan NS, Dejelo C, Boehm BO, Hering BJ, Heeger PS.
American Journal of Transplantation. 2002, 2, 857.

Assay to Assay Reproducibility of Ex Vivo Measurements on PBMC - Fresh versus Cryopreserved PBMC - ELISPOT for Cells Other Than Human PBMC or Murine Lymph Node/Spleen Cells - Transplantation Immunology -

Cytokine detection by ELISPOT: relevance for immunological studies in type 1 diabetes.

Meierhoff G, Ott PA, Lehmann PV, Schloot NC.
Diabetes/Metabolism Research and Reviews. 2002, 18, 367-380.

Autoimmunity

CD4+ T cells recognizing specific antigen deposited in glomeruli cause glomerulonephritis-like kidney injury.

Radeke HH, Tschernig T, Karulin AY, Schumm G, Emancipator SN, Resch K, Tary-Lehmann M.
Clinical Immunology. 2002, 104, 161-173.

Measuring Th1, Th2, Th17, and Other Effector Lineages - Autoimmunity

Vaccination with tumor peptide in CpG adjuvant protects via IFN-gamma-dependent CD4 cell immunity.

Stern BV, Boehm BO, Tary-Lehmann M.
Journal of Immunology. 2002, 168, 6099-6105.

Working with Peptides and Peptide Pools - Vaccine and Adjuvants - Tumor Immunology -

Vaccination with tumor cells pulsed with foreign peptide induces immunity to the tumor itself.

Schlingmann TR, Rininsland FH, Bartholomae W, Boehm BO, Lehmann PV, Tary-Lehmann M.
Clinical Immunology. 2009, 133, 45-51.

Tumor Immunology -

Comprehensive determinant mapping of the hepatitis C-specific CD8 cell repertoire reveals unpredicted immune hierarchy.

Anthony DD, Valdez H, Post AB, Carlson NL, Heeger PS, Lehmann PV.
Clinical Immunology. 2002, 103, 264-276.

Determinant Mapping/High-throughput Screening - Infectious and Other Diseases -

HIV long-term non-progressors maintain brisk CD8 T cell responses to other viral antigens.

Valdez, H., Carlson NL, Post AB, Asaad R, Heeger PS, Lederman MM, Lehmann PV, Anthony DD.
AIDS. 2002, 16, 1113-1118.

Infectious and Other Diseases -

Indirect IL-4 pathway in type 1 immunity.

Karulin AY, Hesse MD, Yip HC, Lehmann PV.
Journal of Immunology. 2002, 168, 545-553.

Measuring Th1, Th2, Th17, and Other Effector Lineages - Cognate Cytokine Production by T Cells versus Bystander Cytokine Production - Cytokine Coexpression/Two-color ELISPOT Assays -

Production of transforming growth factor-beta 1 (TGF-β1) expression in human monocytes infected with Mycrobacterium Avium at a single cell level by ELISPOT assay.

Aung H, Sherman J, Tary-Lehmann M and Toossi Z.
Journal of Immunological Methods. 2002, 259, 25-32.

ELISPOT for Cells Other Than Human PBMC or Murine Lymph Node/Spleen Cells - Infectious and Other Diseases -

Protective anti-Helicobacter immunity is induced with aluminum hydroxide or complete Freund’s adjuvant by systemic immunization.

Gottwein JM, Blanchard TG, Targoni OS, Eisenberg JC, Zagorski BM, Redline RW, Nedrud JG, Tary-Lehmann M, Lehmann PV, Czinn SJ.
Journal of Infectious Diseases. 2001, 184, 308-314.

Vaccine and Adjuvants - Infectious and Other Diseases -

Immunomodulatory effect of extracorporeal photopheresis after successful treatment of resistant renal allograft rejection.

Baron ED, Heeger PS, Hricik DE, Schulak JA, Tary-Lehmann M, Stevens SR.
Photodermatology, Photoimmunology & Photomedicine. 2001, 17, 79-82.

Transplantation Immunology -

A T cell clone's avidity is a function of its activation state.

Hesse MD, Karulin AY, Boehm BO, Lehmann PV, Tary-Lehmann M.
Journal of Immunology. 2001, 167, 1353-1361

Single-cell Resolution and Frequency - Measurements of Functional T Cell Avidity - Spot Size Distribution - Working with Peptides and Peptide Pools -

Staphylococcal enterotoxin-B-induced lethal shock in mice is T-cell-dependent, but disease susceptibility is defined by the non-T-cell compartment.

Anderson MR, Tary-Lehmann M.
Clinical Immunology. 2001, 98, 85-94.

Infectious and Other Diseases -

Pulmonary mononuclear cell responses to antigens of Mycobacterium tuberculosis in healthy household contacts of patients with active tuberculosis and healthy controls from the community.

Schwander SK, Torres M, Carranza CC, Escobedo D, Tary-Lehmann M, Anderson P, Toossi Z, Ellner JJ, Rich EA, Sada E.
Journal of Immunology. 2000, 165, 1479-1485.

ELISPOT for Cells Other Than Human PBMC or Murine Lymph Node/Spleen Cells - Infectious and Other Diseases -

Granzyme B ELISPOT assay for ex vivo measurements of T cell immunity.

Rininsland FH, Helms T, Asaad RJ, Boehm BO, Tary-Lehmann M.
Journal of Immunological Methods. 2000, 240, 143-155.

Cytotoxicity, Granzyme B, and Perforin ELISPOT - Infectious and Other Diseases -

Comprehensive assessment of determinant specificity, frequency, and cytokine signature of the primed CD8 cell repertoire induced by a minor transplantation antigen.

Heeger PS, Valujskikh A, Lehmann PV.
Journal of Immunology. 2000, 165, 12781284.

Determinant Mapping/High-throughput Screening - Working with Peptides and Peptide Pools - Transplantation Immunology -

Quantification of self-recognition in multiple sclerosis by single-cell analysis of cytokine production.

Pelfrey CM, Rudick RA, Cotleur AC, Lee JC, Tary-Lehmann M, Lehmann PV.
Journal of Immunology. 2000, 165, 1641-1651.

Working with Peptides and Peptide Pools - Autoimmunity

Revisiting tolerance induced by autoantigen in incomplete Freund’s adjuvant.

Heeger PS, Forsthuber TG, Shive C, Biekert E, Genain C, Hofstetter HH, Karulin AY, Lehmann PV.
Journal of Immunology. 2000, 164, 5771-5781.

Cytokine Coexpression/Two-color ELISPOT Assays - Vaccine and Adjuvants - AutoimmunityTransplantation Immunology -

Frequencies of neuroantigen-specific T cells in the central nervous system versus the immune periphery during the course of experimental allergic encephalomyelitis.

Targoni OS, Baus J, Hofstetter HH, Hesse MD, Karulin AY, Boehm BO, Forsthuber TG, Lehmann PV.
Journal of Immunology. 2001, 166, 4757-4764.

Autoimmunity Single-cell Resolution and Frequency -

Direct visualization of cytokine-producing recall antigen-specific CD4 memory T cells in healthy individuals and HIV patients.

Helms T, Boehm BO, Asaad RJ, Trezza RP, Lehmann PV, Tary-Lehmann M.
Journal of Immunology. 2000, 164, 3723-3732

Single-cell Resolution and Frequency - Measuring Th1, Th2, Th17, and Other Effector Lineages - Assay to Assay Reproducibility of Ex Vivo Measurements on PBMC - Infectious and Other Diseases -

Selective peptidomimetic blockers of autoantigen presentation: a novel therapeutic approach to autoimmune disease.

Lehmann PV.
Trends in Pharmacological Sciences. 2000, 21, 79-80.

Autoimmunity

Single-cytokine-producing CD4 memory cells predominate in type 1 and type 2 immunity.

Karulin AY, Hesse MD, Tary-Lehmann M, Lehmann PV.
Journal of Immunology. 2000, 164, 1862-1872.

Measuring Th1, Th2, Th17, and Other Effector Lineages - Cytokine Coexpression/Two-color ELISPOT Assays - Spot Size Distribution -

Pretransplant frequency of donor-specific, IFN-gamma-producing lymphocytes is a manifestation of immunologic memory and correlates with the risk of posttransplant rejection episodes.

Heeger PS, Greenspan NS, Kuhlenschmidt S, Dejelo C, Hricik DE, Schulak JA, Tary-Lehmann M.
Journal of Immunology. 1999, 163, 2267-2285.

Transplantation Immunology -

Prevention of murine EAE by oral hydrolytic enzyme treatment.

Targoni OS, Tary-Lehmann M, Lehmann PV.
Journal of Autoimmunity. 1999, 12, 191-198.

Autoimmunity

Adjuvant-guided type- 1 and type-2 immunity: infectious/noninfectious dichotomy defines the class of response.

Yip HC, Karulin AY, Tary-Lehmann M, Hesse MD, Radeke H, Heeger PS, Trezza RP, Heinzel FP, Forsthuber TG, Lehmann PV.
Journal of Immunology. 1999, 162, 3942-3949.

Determinant Mapping/High-throughput Screening - Vaccine and Adjuvants - Measuring Th1, Th2, Th17, and Other Effector Lineages - Measurements of Functional T Cell Avidity -

Enhanced responses to Mycobacterium tuberculosis antigens by human alveolar lymphocytes during active pulmonary tuberculosis.

Schwander, SK, Torres M, E. Sada, C. Carranza, E. Ramos, Tary-Lehmann M, R. S. Wallis, J. Sierra, and E. A. Rich.
Journal of Infectious Diseases. 1998, 178, 1434-1445.

ELISPOT for Cells Other Than Human PBMC or Murine Lymph Node/Spleen Cells - Infectious and Other Diseases -

Heat shock proteins and experimental autoimmune encephalomyelitis. II: environmental infection and extra-neuraxial inflammation alter the course of chronic relapsing encephalomyelitis.

Birnbaum G, Kotilinek L, Miller SD, Raine CS, Gao YL, Lehmann PV, Gupta RS.
Journal of Neuroimmunology. 1998, 90, 149-161.

Autoimmunity

The fate of T cells in the brain: veni, vidi, vici and veni, mori.

Lehmann PV.
American Journal of Pathology. 1998, 153, 677-680.

Autoimmunity

Shifting T-cell activation thresholds in autoimmunity and determinant spreading.

Lehmann PV, Targoni OS, Forsthuber TG.
Immunological Review. 1998, 164, 53-61.

Autoimmunity

Enzyme-linked immunosorbent assay spot detection of interferon-gamma and interleukin 5-producing cells as a predictive marker for renal allograft failure.

Tary-Lehmann M, Hricik DE, Justice AC, Potter NS, Heeger PS.
Transplantation. 1998, 66, 219.

Measuring Th1, Th2, Th17, and Other Effector Lineages - Transplantation Immunology -

Identification of LFA-1 as a candidate autoantigen in treatment-resistant Lyme arthritis.

Gross DM, Forsthuber T, Tary-Lehmann M, Etling C, Ito K, Nagy ZA, Field JA, Steere AC, Huber BT.
Science. 1998, 281, 703-706.

AutoimmunityInfectious and Other Diseases -

Endogenous myelin basic protein inactivates the high avidity T cell repertoire.

Targoni OS Lehmann PV.
Journal of Experimental Medicine. 1998, 187, 2055-2063.

Measurements of Functional T Cell Avidity - Determinant Mapping/High-throughput Screening - Working with Peptides and Peptide Pools - Autoimmunity

High-resolution characterization of cytokine-producing alloreactivity in naive and allograft-primed mice.

Matesic D, Lehmann PV, Heeger PS.
Transplantation. 1998, 65, 906-914.

Transplantation Immunology -

CpG oligodeoxynucleotides act as adjuvants that switch on T helper 1 (Th1) immunity.

Chu RS, Targoni OS, Krieg AM, Lehmann PV, Harding CV.
Journal of Experimental Medicine. 1997, 186, 1623-1631.

Measuring Th1, Th2, Th17, and Other Effector Lineages - Working with Peptides and Peptide Pools - Vaccine and Adjuvants -

Induction of T helper 2 immunity to an immunodominant allopeptide.

Lehmann PV, D. Matesic, G. Benichou, and Heeger PS.
Transplantation. 1997, 64, 292-296.

Measuring Th1, Th2, Th17, and Other Effector Lineages - Transplantation Immunology -

Determinant spreading of T helper cell 2 (Th2) responses to pancreatic islet autoantigens.

Tian J, Lehmann PV, and D. L. Kaufman.
Journal of Experimental Medicine. 1997, 186, 2039-2043.

Measuring Th1, Th2, Th17, and Other Effector Lineages - Autoimmunity

Modulating autoimmune responses to GAD inhibits disease progression and prolongs islet graft survival in diabetes-prone mice.

Tian J, Clare-Salzler M, Herschenfeld A, Middleton B, Newman D, Mueller R, Arita S, Evans C, Atkinson MA, Mullen Y, Sarvetnick N, Tobin AJ, Lehmann PV, Kaufman DL.
Nature Medicine. 1996, 2, 1348-1353.

Autoimmunity

Immunomodulation by proteolytic enzymes

Lehmann PV.
Nephrology Dialysis Transplantation. 1996, 11, 952-955.

Transplantation Immunology -

Nasal administration of glutamate decarboxylase (GAD65) peptides induces Th2 responses and prevents murine insulin-dependent diabetes.

Tian J, Atkinson MA, Clare-Salzler M, Herschenfeld A, Forsthuber T, Lehmann PV, Kaufman DL.
Journal of Experimental Medicine. 1996, 183, 1561-1567

Autoimmunity

Induction of TH1 and TH2 immunity in neonatal mice.

Forsthuber T, Yip HC, Lehmann PV.
Science. 1996, 271, 1728-1730.

Measuring Th1, Th2, Th17, and Other Effector Lineages - Working with Peptides and Peptide Pools - Vaccine and Adjuvants -

The human immune system in hu-PBL-SCID mice.

Tary-Lehmann M., Saxon A, Lehmann PV.
Immunology Today. 1995, 16, 529-533.

Transplantation Immunology -

T cell cross-reactivity between coxsackievirus and glutamate decarboxylase is associated with a murine diabetes susceptibility allele.

Tian J, Lehmann PV, Kaufman DL.
Journal of Experimental Medicine. 1994, 180, 1979-1984.

Autoimmunity

Anti-SCID mouse reactivity shapes the human CD4+ T cell repertoire in hu-PBL-SCID chimeras.

Tary-Lehmann M, Lehmann PV, Schols D, Roncarolo MG, Saxon A.
Journal of Experimental Medicine. 1994, 180,1817-1827.

Transplantation Immunology -

Limited T cell response to donor MHC peptides during allograft rejection. Implications for selective immune therapy in transplantation.

Benichou G., Fedoseyeva E, Lehmann PV, Olson CA, Geysen HM, McMillan M, Sercarz EE.
Journal of Immunology. 1994, 153, 938-945.

Transplantation Immunology -

The SCID mouse environment causes immunophenotypic changes in human immature T-cell lines.

Uittenbogaart CH, Anisman DJ, Tary-Lehmann M, Vollger LW, Breit TM, Van Dongen JJ, Saxon A.
International Journal of Cancer. 1994, 56, 546-551.

Tumor Immunology -

Spontaneous loss of T-cell tolerance to glutamic acid decarboxylase in murine insulin-dependent diabetes.

Kaufman DL, Clare-Salzler M, Tian J, Forsthuber T, Ting GS, Robinson P, Atkinson MA, Sercarz EE, Tobin AJ, Lehmann PV.
Nature. 1993, 366, 69-72.

Autoimmunity

Dominance and crypticity of T cell antigenic determinants.

Sercarz EE, Lehmann PV, Ametani A, Benichou G, Miller A, Moudgil K.
Annual Review of Immunology. 1993, 11, 729-766.

Autoimmunity

Determinant spreading and the dynamics of the autoimmune T-cell repertoire.

Lehmann PV, Sercarz EE, Forsthuber T, Dayan CM, Gammon G.
Immunology Today. 1993, 14, 203-208.

Autoimmunity

Human mature T cells that are anergic in vivo prevail in SCID mice reconstituted with human peripheral blood.

Tary-Lehmann M., A. Saxon.
Journal of Experimental Medicine. 1992, 175, 503-516.

Transplantation Immunology -

Limited B cell repertoire in severe combined immunodeficient mice engrafted with peripheral blood mononuclear cells derived from immunodeficient or normal humans.

A. Saxon. , E Macy, K Denis, M Tary-Lehmann, O Witte, J Braun
The Journal of Clinical Investigation. 1991, 87, 658-665.

Transplantation Immunology -

Graft-versus-host resistance induced by class II major histocompatibility complex-specific T cell clones.

Lehmann PV, K. Drexler, Tary-Lehmann M, F. Falcioni, U. Hurtenbach, and Nagy ZA.
Journal of Experimental Medicine. 1991, 173, 333-341.

Transplantation Immunology -

Induction of graft versus host-associated immunodeficiency by CD4+ T cell clones.

Tary-Lehmann, M., A. G. Rolink, Lehmann PV, Nagy ZA, and U. Hurtenbach
Journal of Immunology. 1990, 145, 2092-2098.

Transplantation Immunology

Natural T cell autoreactivity to melanoma antigens: clonally expanded melanoma-antigen specific CD8 + memory T cells can be detected in healthy humans.

Przybyła A., Zhang T., Li R., Roen D.R., Mackiewicz A., and P. V. Lehmann (2018)
Cancer Immunol. Immunotherapy 2019

Spreading of T-cell autoimmunity to cryptic determinants of an autoantigen.

Lehmann PV, Forsthuber T, Miller A, Sercarz EE.
Nature. 1992, 358, 155.

Autoimmunity

Flexibility of the T cell repertoire. Self tolerance causes a shift of T cell receptor gene usage in response to insulin.

Falcioni F, Dembic Z, Mulller S, Lehmann PV, Nagy ZA.
Journal of Experimental Medicine. 1990, 171, 1665-1681.

Autoimmunity

Acute lethal graft-versus-host reaction induced by major histocompatibility complex class II-reactive T helper cell clones.

Lehmann PV, G. Schumm, D. Moon, U. Hurtenbach, F. Falcioni, Mulller S, and Nagy ZA.
Journal of Experimental Medicine. 1990, 171, 1485-1496.

Transplantation Immunology -

Inhibition of T cell response with peptides is influenced by both peptide-binding specificity of major histocompatibility complex molecules and susceptibility of T cells to blocking.

Lehmann PV, Cardinaux F, Appella E, Muller S, Falcioni F, Adorini L, Nagy ZA.
European Journal of Immunology. 1989, 19, 1071-1077.

Working with Peptides and Peptide Pools -

Why peptides? Their possible role in the evolution of MHC-restricted T-cell recognition.

Nagy ZA, Lehmann PV, Falcioni F, Muller S, Adorini L.
Immunology Today. 1989, 10, 132-138

Working with Peptides and Peptide Pools -

In vivo competition between self peptides and foreign antigens in T-cell activation.

Adorini L, Mulller S, Cardinaux F, Lehmann PV, Falcioni F, Nagy ZA.
Nature. 1988, 334, 623-625.

Working with Peptides and Peptide Pools -

Interaction of an immunodominant epitope with Ia molecules in T-cell activation.

Adorini L, Sette A, Buus S, Grey HM, Darsley M, Lehmann PV, Doria G, Nagy ZA, Appella E.
Proceedings of the National Academy of Sciences. 1988, 85, 5181-5185.

Working with Peptides and Peptide Pools

Quantitative HLA-class-II/factor VIII (FVIII) peptidomic variation in dendritic cells correlates with the immunogenic potential of therapeutic FVIII proteins in hemophilia A.

Diego VP, Luu BW, Hofmann M, Dinh LV, Almeida M, Powell JS, Rajalingam R, Peralta JM, Kumar S, Curran JE, Sauna ZE, Kellerman R, Park Y, Key NS, Escobar MA, Huynh H, Verhagen AM, Williams-Blangero S, Lehmann PV, Maraskovsky E, Blangero J, Howard TE.
J Thromb Haemost 2020,18, 201-216.

IL-21 in Conjunction with Anti-CD40 and IL-4 Constitutes a Potent Polyclonal B Cell Stimulator for Monitoring Antigen-Specific Memory B Cells.

Fridolin Franke, Greg A. Kirchenbaum, Stefanie Kuertenand Paul V. Lehmann.
Cells 2020, 9, 433.

No Results Found