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CTL introduces new HDR imaging platform for FluoroSpot analysis

Conventional FluoroSpot relies on the analysis of a single image (for each channel in multi-color assays) of a well, captured using a single exposure level. While more than adequate for accurate counts when the intensities of the spots to be analyzed are within a normal range, this approach may miss very faint spots, and overly bright spots will most likely be over-exposed, with details of the intensity profile lost from the image.

In HDR imaging, a series of incremental exposure times is used for the well images captured (shown in the video to the left).

Spots at both the low and high ends of the intensity range are captured, at exposure times optimal for accurate detection of each, and thus the full dynamic range of relative spot intensities is captured.

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Fluorescence-capable ImmunoSpot® analyzers now come with high-throughput live/dead/apoptotic PBMC counting capability

Serial Measurements of Apoptotic Cell Numbers Provide Better Acceptance Criterion for PBMC Quality than a Single Measurement Prior to the T Cell Assay

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ImmunoSpot® analyzers now permit measuring cell-mediated cytotoxicity via the Target cell Visualization Assay

High-Throughput GLP-Capable Target Cell Visualization Assay for Measuring Cell-Mediated Cytotoxicity

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Multi-color ImmunoSpot® can accomplish what flow cytometry has been struggling to do

The cytokine co-expression profile of individual T cells determined by multicolor ImmunoSpot® analysis permits to define T cell lineages and activation states. For our recent publications, see:

Natural T cell autoreactivity to melanoma antigens: clonally expanded melanoma-antigen specific CD8 + memory T cells can be detected in healthy humans.

Detection of Antigen-Specific T Cell Lineages and Effector Functions Based on Secretory Signature.

Four Color ImmunoSpot® Assays for Identification of Effector T Cell Lineages.

Spot peak assessment does not suffice to accurately determine cytokine co-expression

Multiplexing T and B Cell FLUOROSPOT Assays: Experimental Validation of the Multi-Color ImmunoSpot® Software Based on Center of Mass Distance Algorithm.

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CTL scientists highlight ImmunoSpot®’s suitability for high-throughput immune monitoring

This recent publication details how comprehensive ImmunoSpot® testing against all potential determinants of an antigen is technically feasible, and calls into question the usage of single proteins or peptides for reliable immune monitoring.

Comprehensive Evaluation of the Expressed CD8+ T Cell Epitope Space Using High-Throughput Epitope Mapping

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New ImmunoSpot® Release: SpotStat™ Software for the statistical analysis of ELISPOT data

Our SpotStat® software suite implements a number of powerful parametric and non-parametric statistical methods for easy, streamlined analysis of data generated by the CTL ImmunoSpot® platform.

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Recent additions to our multi-color ImmunoSpot® portfolio: Seven color Human B cell, and triple color mouse IFN-γ/TNF-α/IL-2 FluoroSpot

Detecting all Immunoglobulin Classes and Subclasses in Multiplex 7 Color ImmunoSpot® Assay.

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Cellular Technology Limited ranked among top 10 image analysis solution providers for 2019

According to the Image Analysis Special edition of Pharma Tech Outlook Magazine, Cellular Technology Limited is the industry leader in the development, use, and manufacture of standardized tools for cellular immune assays.

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GHP announces CTL winner of 2018 Technology Award

“Best Cellular Immune Assays Manufacturing Company 2018 & Leading Provider of Elispot & FluoroSpot Assays 2018”

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